Edvotek 255 Purification, Size Determination of Blue and Green Fluorescent Proteins

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Edvotek 255 Purification, Size Determination of Features

• For 6 lab groups

• Packing and running column 45 minutes, optional electrophoresis 60 minutes, staining 30 minutes, destaining 2 hours

• Kit includes: instructions, columns and matrix, GFP and BFP extracts, buffer, protein gel reagents for optional activity

• All you need: waterbath, long wave UV lamp, ring stand and clamps, automatic micropipet, vertical gel electrophoresis apparatus, power supply, polyacrylamide gels (12 percent)

• In this experiment, the unique fluorescent properties of GFP and BFP will be used as an assay during their purification from an E

About Edvotek 255 Purification, Size Determination Of

When bacteria are used to make medicinally useful proteins by transformation, the protein of interest must be separated from all of the other cellular proteins. In this experiment, the unique fluorescent properties of GFP and BFP will be used as an assay during their purification from an E. coli extract. The column fractions containing GFP or BFP will be identified by fluorescence and then purified. As an optional activity, purified protein fractions can be separated by SDS polyacrylamide gel electrophoresis (SDS-PAGE) to estimate the purity and size of the GFP and BFP proteins. Kit includes: instructions, columns and matrix, GFP and BFP extracts, buffer, protein gel reagents for optional activity. All you need: waterbath, long wave UV lamp, ring stand and clamps, automatic micropipet, vertical gel electrophoresis apparatus, power supply, polyacrylamide gels (12 percent).