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For propagation and maintenance of E. Coli. Low Salt Formula. Molecular Biology Grade. To prepare media, dissolve 20 grams in 1 liter distilled water. Next, adjust the pH of the solution to 7.2. Heat and frequently agitate to completely dissolve. Sterilize by autoclaving for 15 minutes. Ingredients: Tryptone: 10 g/L Yeast Extract: 5 g/L Sodium Chloride: 5 g/L Specifications: DCM Appearance: Homogeneous free flowing, light beige PPM Appearance: Clear, No to light ppt, light yellow pH (2% water) @25°C: 6.8 - 7.2 Microorganism (E. coli ATCC 23724): Good to Excellent Microorganism (E. coli ATCC 33694): Good to Excellent Microorganism (E. coli ATCC 33849): Fair to Excellent Microorganism (E. coli ATCC 39403): Good to Excellent Microorganism (E. coli ATCC 47014): Good to Excellent Microorganism (E. coli ATCC 53868): Good to Excellent Sodium Chloride: 5 g/l Yeast Extract: 5 g/L Casein Peptone: 10 g/L The RPI brand has been serving the scientific community since 1970. Our ISO 9001:2015 and ISO GMP certifications are a testament to our dedication to quality.