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R2A Agar is a culture medium developed to study bacteria which normally inhabit potable water. These bacteria tend to be slow-growing species and would quickly be suppressed by faster-growing species on a richer culture medium. Since its development in 1985, it has been found to allow the culturing of many other bacteria that will not readily grow on fuller, complex organic media. R2A Agar is a low nutrient medium, and in combination with a lower incubation temperature and longer incubation time, stimulates the growth of stressed and chlorine-tolerant bacteria. Enzymatic Digest of Casein and Animal Tissue provide nitrogenous sources for bacterial growth. Yeast Extract supplies B-complex vitamins and serves as growth enhancer. Dextrose is the fermentable carbohydrate. Soluble starch serves as neutralizer, while Sodium Pyruvate stimulates the growth of stressed bacteria. Dipotassium Phosphate and Magnesium Sulfate Heptahydrate act as pH buffers. Agar is the gelifying agent. The professional packaging allows for an extended shelf-life that is more than double today's standard.