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✔Measurement 1) Place 1-2 drops of the liquid on the liquid carrier of the refractometer 2) Cover the prism (liquid will spread more easily over the prism) 3) Look through the eyepiece against the light (focus if necessary) ✔Calibration Before measure, the standard solution (pure water), instrument and sample must be at the same temperature for accurate results. Place 1-2 drops of the pure water on the liquid carrier of the refractometer. And cover the prism. Turn the calibration screw make the lights and darks boundary line of the field of view of eyepiece and datum line have coincided. (0%) ✔Package Contents 1x refractometer 1x pouch 1x pipette 1x cleaning cloth 1x operating instructions ✔Note 1) For accurate results, please calibrate the refractometer before your first use. 2) After using it, don't wash it with the tap water directly. avoid the water enters the body. clean the instrument with the soft, damp cloth and ensure accurate measurement. 3) Avoid to scratch or collision damages on the surface of the optical components.