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There are only a few days in a woman's cycle when conception can occur, the day prior to and immediately following the day of ovulation. This is why AccuMed's One Step LH Ovulation Test Strips are manufactured to exclusive quality standards and include US warranty and support provided directly by Accumed Biotech. The Luteinizing Hormone (LH) which is in the urine of normal women will increase dramatically in the middle of the menstrual cycle. The Luteinizing Hormone (LH) which is in the urine of normal women will increase dramatically in the middle of the menstrual cycle. The LH increase triggers ovulation which is when the egg is released periodically from normal fertile women. AcuMed's Ovulation Urine Test is a qualitative test used to predict when there is a LH surge, and in turn, when you are likely to ovulate. WHO experts state that LH testing is a reliable way to detect ovulation. Ovulation will occur after 24-48 hours following a positive test.