87785 - Halt Protease Inhibitor Cocktail (100X), Thermo Scientific - Halt Protease Inhibitor Cocktail, EDTA-Free (100X), Sufficient to Treat 100 mL of Sample - Each (1ml)

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About 87785 - Halt Protease Inhibitor Cocktail

Thermo Scientific Protease Inhibitor Cocktail (X) are ready-to-use concentrated stock solutions of protease inhibitors for addition to samples to prevent proteolytic degradation during cell lysis and protein extraction from animal and plant tissues, cultured cells,yeast or bacteria. Convenient No proprietary ingredientsn/afully disclosed formulation Multiple package sizes Regular formulationn/aincludes a separate vial of EDTA solution for optional metalloprotease inhibition Lysis buffer compatibleThe cocktail inhibits serine and cystine proteases, calpain proteases, and metalloproteases Proteases make up a large category of enzymes, including endopeptidases and exopeptidases, which catalyze the hydrolytic breakdown of proteins into peptides or amino acids in a wide variety of cell and tissue types. Proteases are regulated and compartmentalized in living cells, but cell lysis releases and mixes them with other extracted proteins. Their activity can thus lead to the loss of a large number of valuable proteins, adversely affecting downstream applications. For this reason, it is common practice to spike cell lysis buffers or cell extracts with a cocktails of compounds known to inactivate or inhibit proteases. Protease Inhibitor Cocktails effectively inhibit serine-proteases, cysteine-proteases, aspartic acid-proteases and aminopeptidases that are typically present in cellular lysate samples. Regular and EDTA-free formulations provide for inhibition of metalloproteases and compatibility with 2D electrophoresis, respectively. The unique EDTA-Free formulation effectively inhibits most metalloprotease activity while allowing optimal action of nuclease activity for removing nucleic acids from samples.