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Edvotek 112 Laboratory Kit 6 - Analysis of Eco RI Cleavage Patterns of Lambda DNA for 8 Gels
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About Edvotek 112 Laboratory Kit 6 - Analysis Of Eco RI
Description This experiment introduces the use of restriction enzymes as a tool to digest DNA at specific nucleotide sequences. Bacteriophage lambda DNA has a linear structure and 6 Eco RI recognition sites. Separation by agarose gel electrophoresis of an Eco RI digest of lambda DNA will yield 6 bands (5 distinct bands, two are very close in size) corresponding to the DNA fragments. Kit includes: instructions, Ready-to-Load DNA samples, UltraSpec-Agarose powder, practice gel loading solution, electrophoresis buffer, InstaStain Blue and FlashBlue stain, calibrated pipet, 100 ml graduated cylinder, and microtipped transfer pipettes. All you need: electrophoresis apparatus, power supply, automatic micropipet and tips, balance, microwave or hot plate, 65 degree C waterbath, visualization system. DNA samples are stable at room temperature. However, if the experiment will not be conducted within one month of receipt, it is recommended that the DNA samples be stored in the refrigerator. DNA samples do not require heating prior to gel loading. Ready-to-Load DNA samples for electrophoresis: A: Standard DNA Fragments, B: Lambda DNA cut with Eco RI, C: Lambda DNA (uncut).
