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HiMedia Laboratories M070S-2.5KG MR-VP Medium Gluc Phosphate Broth, 2.5 kg
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HiMedia Laboratories M070S-2.5KG MR-VP Medium Gluc Features
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Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label
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Positive test is indicated by eosin pink color within 2-5 minutes
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Weight: 2.5kg
About HiMedia Laboratories M070S-2.5KG MR-VP Medium Gluc
Clark and Lubs found that the addition of methyl red to cultures of Escherichia coli resulted in a red color due to high acidity produced during dextrose fermentation. Vogues-Proskauer (2) reported red coloration after addition of potassium hydroxide to specific culture media with organisms grown in it. The investigators developed MR-VP Broth which enables both tests to be performed in same medium in different tubes. The red color produced by the addition of potassium hydroxide to cultures is due to the ability of organisms to produce a neutral product acetoin (acetyl methyl carbinol) from dextrose (3). The acetoin is oxidized in the presence of oxygen and alkali to produce diacetyl which reacts with creatine to produce a red color. This formulation is also recommended by BIS (5) and ISO committee (8) for the detection of coli-aero genes group. A slightly modified formulation (M070S) is recommended by BIS (4,6,7) for the detection of E. coli, Vibrio parahaemolyticus and Bacillus cereus responsible for food poisoning. To test V. Parahaemolyticus for VP, addition of 2-3% Sodium chloride to the medium is required. The Methyl Red (MR) test is performed after maximum of 5 days of incubation at 30°C (9) and Vogues-Proskauer test (VP) cultures are incubated at 30°C for 24-48 hours (10). Various other tests have been suggested by Werkman (11), O'Meara (12) Levine, Epstein and Voughn (13) and Voughn, Mitchell and Levine (9). Werkmans Test (8): Add 2 drops of a 2% solution of ferric chloride to 50 ml culture and 5 ml of 10% sodium hydroxide. Shake the tube to mix well. Stable copper color developing in a few minutes is positive reaction. O'Meara Test (8): Add of 25 mg of solid creatine to 5ml culture and then add 5 ml concentrated (40%) sodium hydroxide. Red color development in a few minutes after shaking the tube well, is a positive reaction.
