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The Rapid FCOV Ag Test Kit is one-step diagnostic reagent based on the immunochromatographic assay of the FCOV antigen in feline conjunctiva fluid.A specific antibody to FCOV is conjugated with gold particles and another is immobilized on the nitrocellulose membrane. If FCOV antigen is present in the specimen, the antibody- gold conjugates will bind to it forming antibody- antigen complex. As the mixture flows along the membrane, the complex is captured on the test line (T) of the membrane, producing a visible read band. Another gold-conjugated reagent is captured by the antibody immobilized on the control line (C) of the membrane. Otherwise, the T line does not develop color. The control line (C) develops color regardless of the presence of FCOV antigen.