Coomassie Brilliant Blue R-250 (CBBR) Analytical Dye from Cepham Life Sciences - 100 g

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Coomassie Brilliant Blue R-250 (CBBR) Analytical Features

• Each production lot is manufactured following stringent Good Manufacturing Practices and rigorous quality control tests

• Product is guaranteed to perform as per its SOP and is backed with a money back guarantee

• Bagged bottle with printed lot number

About Coomassie Brilliant Blue R-250 (CBBR) Analytical

Coomassie Brilliant Blue R-250 is a common form of coomassie dye, which is a key component of various colorimetric protein gel stains. Coomassie R-250 and G-250 dyes are two chemical forms of a disulfonated triphenylmethane compound that is commonly used as the basis of stains for detection of proteins in gel electrophoresis and Bradford-type assay reagents for protein quantitation. The R-250 (red-tinted) form lacks two methyl groups that are present in the G-250 (green-tinted) form, which is also called colloidal coomassie dye. Typically, coomassie gel stains and protein assay reagents are formulated as very acidic solutions in 25 to 50% methanol. In acidic conditions, the dye binds to proteins primarily through basic amino acids (primarily arginine, lysine and histidine), and the number of coomassie dye ligands bound to each protein molecule is approximately proportional to the number of positive charges found on the protein. Protein-binding causes the dye to change from reddish-brown to bright blue (absorption maximum equals 595 nm). Synonym: Brilliant Blue R CAS No.: 6104-59-2 Molecular Formula: C45H44N3O7S2Na Molecular Weight: 826 Appearance: Dark purplish, bluish brown powder Solubility: Dark blue-black solution (10 mg/mL in Water) Storage temp.: Store at RT Application/Features: Easy detection; develops intensely colored complexes with proteins High sensitivity; can determine as little as 0.5 µg/cm2 of protein present in a gel matrix Reversible staining; anion of Coomassie Brilliant Blue formed in the acidic staining medium combines with the protonated amino groups of proteins by electrostatic interaction; resulting complex is reversible under the proper conditions Differentiation between bound and unbound dye; when dissolved in 0.01M citrate buffer at pH 3.0, has an absorption max at 549nm.