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L-Proline is one of the main amino acids of collagen and may support healthy joints and healthy musculoskeletal system. Collagen is a major structural component of cells. It is the protein responsible in forming connective fibers in tissues such as skin, ligaments, cartilage, bones and teeth. Collagen production decreases significantly with age and is the source of a number of unhealthy conditions. L-Proline, L-Lysine and Ascorbic acid all are essential for the formation of collagen and cartilage. Though L-Proline can be made in the body, L-Proline supplementation is necessary for aging and low protein diet and vegetarian diet people. Generally Suggested Use: For adult, to take 1 flat level teaspoon of this powder (about 2000mg) mix in water or juice, 1 to 2 times daily; Or do your own research for use amount, and your own usage purpose.